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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and therefore may well contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a important job in advertising and marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two more splice variants had been reported but specific nucleotide sequence details is not however out there.

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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide typically incorporates the epitope regarded via the antibody.

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This guideline outlines even more achievable optimization of cellular and immunoassay parameters to guarantee the absolute best effects are...

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This manual outlines more achievable optimization of mobile and immunoassay parameters to make sure the best possible final results are...

Antibodies certain to the blocking peptide not bind for the epitope within the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, you can see which staining is distinct.

Target Information Cyclin E belongs to the highly conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E types a complex with and functions like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...

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